5月31日(火)「Modern SPRI: Single Nanoparticle SPR Imaging Measurements of Bioaffinity, Uptake and Surface Enzymatic Reactions」講師:Prof. Robert M. Corn

先導研研究者の皆様

下記の通り、日本表面科学会と共催でUC-IrvineのRob Corn先生の講演会を開催します。参加費は無料です。ご興味のある方はご参集ください。
講演会ポスターへ

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          九州大学先導研講演会

共 催 : 日本表面科学会(ソフトナノテクノロジー部会・九州支部)
日 時 : 2016年5月31日(火)15:00-16:30
場 所 : 九州大学 伊都キャンパス 先導物質化学研究所(CE41)1階 第一会議室

講演タイトル :
「Modern SPRI: Single Nanoparticle SPR Imaging Measurements of Bioaffinity, Uptake and Surface Enzymatic Reactions」

講 師 :  Prof. Robert M. Corn(Department of Chemistry, University of California-Irvine, USA)

要 旨
Surface plasmon resonance imaging (SPRI) is a multiplexed method for the detection of protein and nucleic acids used by researchers around the world. By incorporating nanoparticles and surface enzyme chemistries into SPRI measurements, we have been able to detect DNA, RNA and protein biomarkers at extremely low (femtomolar) concentrations. By using near infrared SPRI microscopy to detect the adsorption of single nanoparticles in real time, we can increase the sensitivity of SPRI measurements even further.[1,2]  In this talk, I will explain how to use single nanoparticle SPRI microscopy to: (i) obtain digital adsorption binding curves for the ligation capture and detection of single microRNA molecules at femtomolar concentrations, and (ii) measure the specific uptake of peptides and proteins into single NIPAM hydrogel nanoparticles.

1.  Aaron R. Halpern, Jennifer B. Wood, Yong Wang and Robert M. Corn, "Single Nanoparticle Near-Infrared Surface Plasmon Resonance Microscopy for Real-Time Measurements of DNA Hybridization Adsorption" ACS Nano, 8 1022-1030 (2014).
2. Kyunghee Cho, Jennifer B. Fasoli, Keiichi Yoshimatsu, Kenneth J. Shea and Robert M. Corn,"Measuring Melittin Uptake into Hydrogel Nanoparticles with Near-Infrared Single Nanoparticle Surface Plasmon Resonance Microscopy"
Analytical Chemistry

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連絡先:玉田 薫



Posted:2016/05/20.